Week 1 - September 6, 2016
| 2016 NJCAA Football Poll - Week 1 | |||||
| Rank | Team | Record | Points | 1st Place Votes | Prev. Rank |
| 1 | Trinity Valley | 2-0 | 137 | 5 | 3 |
| 2 | Snow | 2-0 | 136 | 4 | 2 |
| 3 | Butler (KS) | 1-0 | 119 | 0 | 5 |
| 4 | Copiah-Lincoln | 1-0 | 114 | 0 | 7 |
| 5 | Arizona Western | 2-0 | 111 | 0 | 12 |
| 6 | Northwest Mississippi | 1-0 | 107 | 0 | 10 |
| 7 | Hutchinson | 2-0 | 99 | 0 | 16 |
| 8 | Tyler | 1-0 | 83 | 0 | 13 |
| 9 | Lackawanna | 1-0 | 82 | 0 | 11 |
| 10 | DuPage | 2-0 | 72 | 0 | 14 |
| 11 | Itawamba | 1-0 | 58 | 0 | NR |
| 12 | East Mississippi | 0-1 | 53 | 0 | 1 |
| 13 | Navarro | 1-1 | 45 | 0 | 9 |
| 14 | Garden City | 2-0 | 39 | 0 | NR |
| 15 | Jones | 1-0 | 36 | 0 | NR |
| 16 | Eastern Arizona | 2-0 | 27 | 0 | RV |
| 17 | Mississippi Gulf Coast | 0-1 | 25 | 0 | 4 |
| 18 | Glendale | 2-0 | 23 | 0 | NR |
| 19 | North Dakota SCS | 2-0 | 18 | 0 | NR |
| 19 | Dodge City | 2-0 | 18 | 0 | NR |
Receiving Votes: Georgia Military, Iowa Western, Coffeyville, New Mexico Military, Kilgore, Pima, Central Lakes, Blinn